The one-leg tie in Duisberg has potential to be the pick of the round with both sides genuine contenders to go all the way.
The five-time Europa League winners have already locked up a place in next season’s Champions League through a top four finish in La Liga.
While Wolves just missed out and will need to win the competition in order to play European football next season.
Here are the predicted line-ups and team news for the game.
When is it and what time is kick-off?
The match will begin at 8pm BST on Tuesday 11 August at the MSV Arena in Duisberg, Germany.
How can I watch it online and on TV?
The game will be broadcast live on BT Sport 1 and BT Sport Extra 1, as well as BT Sport Ultimate. Coverage starts at 7pm BST.
Subscribers can also stream the match on the BT Sport website and app.
Wolves XI: Boly, Coady, Saiss; Doherty, Neves, Dendoncker, Moutinho, Vinagre; Traore, Jimenez
Sevilla XI: Bono; Jesús Navas, Koundé, Diego Carlos, Reguilón; Fernando, Jordán, Banega, Ocampos, Suso; En-Nesyri
Wolves – 13/5
Draw – 23/10
Sevilla - 21/20
Sevilla have more experience at this level, but Nuno’s Wolves are very capable of going all the way and the Spanish side represent the toughest possible test. Julen Lopetegui up against the Portuguese boss represents a fascinating battle, which is where this game is likely to be won. Podence will be a big loss for the Premier League side, but we’ll lean towards the upset here with their discipline and physicality blending well with several match-winners, such as Traore, Neves or Jimenez, capable of producing a moment of magic. 2-1 Wolves.