Thousands of Victorian senior students will sit the revamped General Achievement Test.
The revised GAT will assess a student's literacy and numeracy skills against new standards, while skills in English, mathematics, science, technology, the arts and humanities will also be assessed.
Students completing the Victorian Certificate of Applied Learning (VCAL) will also sit the GAT for the first time.
More than 89,000 students are expected to complete the test across the state on Wednesday morning.
"The GAT is an important test that ensures VCE assessments are fairly and accurately assessed across the state," Victorian Curriculum and Assessment Authority chief executive Stephen Gniel said.
"Now, with the introduction of the literacy and numeracy skills assessment, it will play an even more important role for more senior secondary students across VCE and VCAL."
GAT results do not contribute to a student's Australian Tertiary Admission Rank (ATAR) unless the student cannot complete their end-of-year exams or assessments.
The test results will be issued in December as part of the student overall results package.
The changes to this year's GAT are part of broader reforms to Victoria's senior secondary education system.
Under the reforms, the senior VCAL system will be replaced with a VCE Vocational Major program from next year.
Intermediate VCAL will also be swapped out for the Victorian Pathways Certificate.
The changes will give students better support and flexibility to study what they want, the Victorian government says.