A Prahran bar has caused uproar after bouncers allegedly refused entry to a gay couple holding hands and told one woman accompanied by her girlfriend to 'put on a dress'.

Furious patrons have taken to restaurant review website Urban Spoon accusing bouncers at Hoo Haa Bar on Chapel Street of discriminating against gay couples.

One patron posted: "I am horrified that on Saturday night whilst eating dinner I overheard and witnessed a young lady be discriminated against for holding her gf hand whilst trying to enter the premises for dinner. I was so disgusted that I up and left."

Another patron claimed a bouncer refused entry to a woman holding her girlfriend's hand before advising her to go home and 'put on a dress'.

"Hoo Haa bar advised my beautiful, gay, neatly dressed, sober friend that she was not allowed in essentially because she was a lesbian. She was told to go home and put a dress on amongst other hateful comments as she showed up holding her girlfriend's hand."

Co-owner Paul Kasteel has flatly denied his business is discriminatory but said he would investigate the claims, posted to the website on July 12.

He told News Ltd: "As a business that's been operating for seven years, we've encouraged a diverse range of people to come to our venue.

"We've been a sponsor for the Melbourne Queer Film Festival for the last three years...we employ gay and straight people, we don't discriminate against staff, why would we discriminate against anyone else?"

Latest From 7News