Michael Clarke jogged several laps of the oval during Australia's warm-up game in Chennai on Wednesday in an encouraging sign that the skipper can overcome a sore hamstring for the first Test from February 22.
Clarke has been rested from the opening two-day game in which the tourists were bowled out for 241 on Tuesday and the India Board XI reached 3-61 at lunch on day two.
The form of offspinner Nathan Lyon (1-26 off seven overs) and pace-bowling allrounder Moises Henriques (2-1 off three overs) were also positive notes for the tourists.
Uncapped offspinner Parvez Rassol claimed 7-45 in Australia's first dig and the pitch was certainly offering some turn on Wednesday.
Lyon made the initial breakthrough for the tourists on day two, in the first over after drinks.
Robin Uthappa was brilliantly caught by Glenn Maxwell at mid-wicket for 24 at 1-37.
Henriques struck with his second delivery when Mandeep Singh was caught behind attempting a hook shot on three.
Henriques also rattled the stumps of opener Abinhav Mukund for 21 with a ball that appeared to keep low.
Ambati Rayudu (10) and Kedar Jadhav (one) are not out at lunch.
Australia's entire 17-man squad has assembled in Chennai after completing commitments with the one-day series at home against West Indies.
Allrounder Shane Watson, who is planning to play as a batsman only on the Indian tour following injury problems, had a solid batting session in the nets on Wednesday.
Clarke performed a series of half-paced run-throughs around the oval under supervision from team doctor Peter Brukner and physio Alex Kountouris.
The fitness of Clarke and opener David Warner (thumb) will be assessed ahead of the three-day game against India A which starts on Saturday.