More than a century ago, the old Munster quarantine station was the scene of hundreds of deaths from contagious diseases such as bubonic plague and smallpox.
The station, at Woodman Point, was built in 1886 to isolate and cremate victims of diseases that were once major causes of death.
Perth medium Rebecca Millman, 38, claims the spirits of many of those who died there are confused about their premature deaths and have refused to cross over.
Each year, she holds a series of overnight ghost hunts at the historic landmark - a tour she says is not for the fainthearted.
"At this site people have lost their lives because of an illness so the spirits there are more willing to communicate with you and they're not aggressive," Ms Millman said.
"The level of activity we got when we first went to the site was phenomenal."
Ms Millman, who has run ghost hunts at the station for three years, said tour members, who slept in one of the old hospital buildings, often heard footsteps and groaning, saw spirit lights and one woman's glasses had been knocked off her nose.
She said others had reported equipment such as cameras not working as they should, changing room temperatures or feeling a physical touch.
They used paranormal measuring equipment to gauge the spiritual presence at different sites within the station.
"I usually find that we get two types of people - those who already have a belief in these things, so they know what to expect, and those who are more sceptical and want to see proof that there's more out there," she said.
"People often hear my name being called and we get things like hearing running water when there's no water running at all.
The annual overnight hunts will be held on Friday and Saturday from 7.45pm to 10am.