Australian Rolf Harris and Susan Boyle will perform at the Queen's Diamond Jubilee Pageant - with Dame Helen Mirren among a list of stars introducing the acts.
The Queen and the Duke of Edinburgh will attend the event, in the private grounds of Windsor Castle, on Sunday, May 13.
More than 550 horses and 1100 performers will be involved in the 90-minute theatrical show, highlighting "the Queen's passion for horses and everything equestrian".
Along with Dame Helen, who famously won an Oscar for her film role as the Queen, Doc Martin star Martin Clunes, The Kumars' Sanjeev Bhaskar and comedian Omid Djalili will introduce acts on stage.
The event, which will also feature military displays, horses and dancers, celebrates more than 250 Commonwealth and state visits that the Queen has made during her reign.
Vocal quartet Il Divo, US classical violinist David Garrett, Nashville singer and banjo player Abigail Washburn, Indian folk star Raghu Dixit and South African actors from the West End musical Lion King, who will be joined by the Nairobi Chamber Chorus and Watoto Children's Choir, have also been booked to perform.
"We have been overwhelmed by the goodwill and support from stars across the globe, who each have their own personal memories of the Queen, and share an enormous amount of love and respect for her work," the show's director and producer Simon Brooks Ward said.
"The Diamond Jubilee Pageant will reflect the truly global impact the Queen has had during her 60-year reign by bringing horses, people, music and their culture from around the world to join the party.
"When you think of all the state occasions the Queen has been involved with, horses have always been there. The Queen's passion for horses is one that the public recognise as her most treasured."
Boyle, who sang a cover of Rolling Stones track Wild Horses, and Harris, famous for his legendary wobble board and didgeridoo, will perform separately on the Sunday, when the Queen and the Duke will attend.
Money raised from ticket sales for the event, which runs from May 10 to May 13 and will be attended by members of the Royal Family throughout, will go towards charities, which are yet to be announced.