Work is continuing at the Carmichael coal mine and rail project after police removed protesters trying to blockade the site in central Queensland.
The protest by a group of 15 people is the second launched against mine project owned by Bravus, previous known as Adani Australia, in three days.
Bravus is building a 10 million tonne-a-year thermal coal mine in the Galilee Basin, which could eventually be expanded to six times that size.
The company is also building a rail line that will be opened to other companies if it gets the tick of approval.
Public servant Carine Visschers tried to block vehicles while holding a sign saying "Make Ecocide A Crime" at a worksite entrance near Mount Coolon on Friday morning.
"Ecocide is mass damage and destruction of ecosystems - harm to nature which is widespread, severe and systematic," the 63-year-old said in a statement.
"Our justice system puts 10-year-old children in prison for damaging property, but enables billionaires like Adani (Bravus) to increase their fortune damaging our land and ecosystems and destroying our children's future.
"We need to make ecocide a crime to have the hope of a liveable future."
Ms Visschers said preventing "ecocide" and climate change depended upon individuals taking action to stop "destructive projects like Adani."
Bravus said work at the Mount Cooton site was not affected by Friday morning's protest.
"Police attended and the protest was finished by 9am with the road cleared for movement," a spokesperson told AAP.
It's the second time demonstraters have tried to blockade the Carmichael project sites this week.
A group of 20 health workers and academics tried to block vehicles entering a rail construction site off the Gregory Hwy on Wednesday morning.
Calling themselves Health On The Frontlines they said they were protesting the impacts of coal mining and climate change on human health.