The new Royal Adelaide Hospital will finally open on September 5.
Health Minister Jack Snelling announced the date, on which the new hospital's Emergency Department will start taking patients from 7am, at the site on Monday morning.
The $2 billion project has been dogged by controversy with its opening repeatedly delayed.
Mr Snelling says about 300 patients at the existing hospital will be shifted across to the new facility in ambulances over three days, starting from September 4.
About another 300 patients will already have been moved from the old RAH to other hospitals in Adelaide in the six weeks leading up to the shift.
Some services that do not require an overnight stay, like radiation oncology and various outpatient clinics, will be offered at the new RAH from mid-August.
Mr Snelling says the opening of the new facility will be a "game changer" for healthcare in SA.
"This is the world's best hospital," he said in a statement on Monday.
"In just a few short months the expanded services, advanced technology and improvement to the delivery of patient wellbeing will speak entirely for itself."
Premier Jay Weatherill said the opening will be "one of the most significant events in out great state's history".
The group representing SA's nurses and midwives said it is significant that those working at the RAH now have "a solid date to work towards".
"Particularly for the more than 2000 nursing staff who have been preparing for the transition to the new facility for some years now," Australian Nursing and Midwifery Federation SA's secretary Elizabeth Dabars said.
But Professor Dabars said there is still work to be done to prepare for the move.
"Testing and remedial work continues to be undertaken and until those processes are complete, and nursing staff are able to visit and review their practice in the new environment, we may still face further obstacles," she said.