New Zealand arrivals to Queensland will have to undergo quarantine from 6pm tonight due to increased transmission across the Tasman.
The "green flights" allowing for unrestricted travel will cease and New Zealand will be removed as a safe travel country.
Chief Health Officer Jeannette Young said the move was needed to protect Queensland.
"Unfortunately, over the past few days New Zealand has been recording some new positive cases, which is why we are closing the border from 6pm," she said.
"Anyone who is permitted to travel to Queensland from New Zealand will be required to quarantine on arrival."
Authorities are asking all travellers who have been in New Zealand since February 21 to get tested and isolate until they get their results.
This also applies to anyone who has entered from February 6 who has not already had a test after entering Queensland.
"New Zealand is working very hard to contain this community transmission, but they are still in a critical stage of their response so we need to keep watching closely," Dr Young said.
"Things can change really quickly with this virus, we've seen that in other countries around the world, which is why I am asking Queenslanders to reconsider their need to travel to New Zealand."