The Queensland government is being urged to intervene to ensure Bravus' controversial coal mine project is not draining a nearby nationally significant wetland.
About 100 protesters rallied outside state parliament to call for the government to investigate the Carmichael mine and rail project in central Queensland.
The $2 billion project is being built by Indian-owned Bravus Mining and Resources, formerly known as Adani Australia.
Wangan and Jagalingou leader Adrian Burragubba says the project is threatening the water table and the nearby Doongmabulla Springs, which is listed by the Australian government as an important wetland.
"Adani's coal mine will drain the life out of the land and destroy our dreaming and the sacred Doongmabulla Springs," he said in a statement.
"It will be a catastrophe every bit as destructive to our culture, and as hurtful to our people, as the blasting of the caves at the Juukan Gorge (in Western Australia)."
Mr Burragubba called on the state government to order work to stop so there can be an independent investigation of the impact on the wetland.
Land and water, he said, is inseparable from indigenous culture and extracting groundwater locks in future environmental damage.
"We have raised many times that the Adani mine breaches our human rights, as there was no free, prior, and informed consent for the issuing of the mining leases and that our lands and waters are in peril," he said.
"The Queensland government and Adani's investors are responsible for the terrible impacts occurring on our country and to our sacred sites and they must respect our human rights."
Bravus is building a 10 million tonne-a-year capacity thermal coal mine in the Galilee Basin, which could be expanded to six times that size.
It is also building a rail line that will be opened to other companies if it gets the tick of approval to mine coal in the region.