More than 100 people were forced to evacuate a University of Melbourne building after water from a sprinkler system mixed with cleaning chemicals.
Emergency crews were called to the campus on Grattan St, Parkville, just after midday on Tuesday following reports of a possible chemical spill.
Fire Rescue Victoria said about 20 firefighters, including specialist hazmat crews, quickly responded before it was declared under control about 2.15pm.
"FRV crews were called to the incident after reports of a possible chemical spillage in a washroom of a research facility," a spokeswoman said.
"Firefighters arrived on scene in six minutes and worked with FRV scientific officers to contain the spillage and make the scene safe."
A university spokesman said the sprinkler set-up caused water damage in a part of the Kenneth Myer Building that houses the Florey Institute.
"Water from the sprinkler system has mixed with cleaning materials in a washing facility on the 6th floor," he told AAP.
"Investigations are underway to determine how the sprinkler was activated."
FRV said more than 100 people were evacuated from the site but the university said it was a precautionary measure, with no reports of injuries.
The Kenneth Myer Building is expected to reopen on Wednesday.