Public school teachers in Katherine will walk off the job in protest over a pay dispute, mounting work pressures, and concerns over inadequate housing.
While schools will remain open to receive students, Australian Education Union members will strike from 9am until 1pm on Wednesday.
The action comes after the union's Northern Territory executive recently voted not to endorse a revised pay offer it believes would leave NT teachers inadequately paid and overworked.
Branch president Michelle Ayres said teachers in Katherine have been dealing with shocking housing conditions.
"We are asking the government to engage these members and hear their stories. This is about more than a few calculations coming out of Treasury," she said.
"This is about people's lives - the lives of the teachers and the impact on the students."
Katherine Regional Council president Rodney Gregg said many long-term teachers, who did not want to leave the region, were receiving attractive offers from interstate.
"With the 2023 school year fast approaching, we would like to know if there will be any incentives offered to stay and teach in Katherine, and what is being done to attract new teachers into the region to replace the ones that have already left," he said.
Education NT said schools would operate as normal on Wednesday.
"Every effort will be made to minimise impacts on learning and to have appropriate supervision in place for students to ensure their safety and wellbeing," it said in a statement.
"Alternative arrangements may be in place during this time, such as modifying timetables and combining classes. Students can anticipate different teachers and staff to supervise them as required."