Israel has restricted its so-called COVID-19 Green Pass to allow only those who have received a vaccine booster dose or recently recuperated from coronavirus to enter indoor venues.
The new criteria mean that nearly two million people will lose their vaccination passport in the coming days.
Israel is the first country to make a booster shot a requirement for its digital vaccination passport.
The move is widely seen as a step to encourage booster vaccination among those who have yet to receive a third dose.
Under the new guidelines, people must have received a booster shot to be eligible for a green pass.
Those who have received two vaccine doses and those who have recovered from coronavirus will be issued passes valid for six months after the date of their vaccination or recovery.
The government's advisory cabinet on coronavirus was set to convene on Sunday to discuss existing restrictions and guidelines.
Technical problems hamstrung the health ministry's roll-out of the updated green pass as millions of Israelis tried to reissue digital documentation that would allow entry to indoor venues.
The green pass is necessary to access almost all areas of public life in Israel: everyone aged three and older must prove that they have been vaccinated, have recovered from a COVID-19 infection or tested negative if they want to visit sporting or cultural events, gyms, museums, restaurants, universities or conferences.
Scores of Israelis staged demonstrations around the country in protest of the green pass system, with convoys of cars clogging morning commutes as many Israelis returned to work on Sunday after September's Jewish High Holidays.
Opponents of the system said it is a form of forced vaccination.
"We are totally against any forced vaccinations or any forced medications and we are totally against doing anything to our children and grandchildren that we don't agree with," said Sarah Felt, who protested along the main highway connecting Jerusalem and Tel Aviv.
Israel raced out of the gate early this year to vaccinate most of its adult population after striking a deal with Pfizer to trade medical data in exchange for a steady supply of doses.
This summer Israel launched an aggressive booster campaign to shore up waning vaccine efficacy in its population.
More than 60 per cent of Israel's population has received two doses of the Pfizer-BioNTech vaccine and nearly 3.5 million of Israel's 9.3 million citizens have received a booster dose of the vaccine.
But at least two million more have received just two doses and many will lose the privileges bestowed by the green pass.
There has been a surge in new cases of coronavirus in Israel in recent months.
As of Sunday, more than 70 per cent of the 588 serious coronavirus cases in Israeli hospitals were unvaccinated individuals, according to health ministry data.
The ministry issued a statement on Sunday morning that because of heavy traffic on its green pass website and app, previously existing certificates would be valid in the coming few days.