Tehran accused Paris on Sunday of "interference" in the case of an Iranian-French academic held in the Islamic republic, saying she is considered an Iranian national and faces security charges.
France said Friday it summoned Iran's ambassador to protest the imprisonment of Fariba Adelkhah and another academic, Roland Marchal of France, saying their detention was "intolerable".
Their imprisonment has added to distrust between Tehran and Paris at a time when French President Emmanuel Macron is seeking to play a leading role in defusing tensions between Iran and its arch-foe the United States.
"The statement by France's foreign ministry regarding an Iranian national is an act of interference and we see their request to have no legal basis," Iran's foreign ministry spokesman Abbas Mousavi said in a statement.
"The individual in question (Adelkhah) is an Iranian national and has been arrested over 'acts of espionage'," he said, adding that her lawyer had knowledge about the details of the case which is being investigated.
Iran does not recognise dual nationality and has repeatedly rebuffed calls from foreign governments for consular access to those it has detained during legal proceedings.
In its statement on Friday, the French foreign ministry reiterated its call for the release of Adelkhah and Marshal.
It also reaffirmed France's demand for consular access.
In response, Mousavi said Marshal was detained for "conspiring against national security", that he has had "consular access multiple times" and that his lawyer was in touch with the judiciary.
- Hunger strikes -
A specialist in Shiite Islam and a research director at Sciences Po University in Paris, Adelkhah's arrest for suspected "espionage" was confirmed in July.
Her colleague Marchal was arrested while visiting Adelkhah, according to his lawyer.
A judge had decided to release the two on bail this month, as they had been entitled to it after six months in detention, their lawyer said.
But this was opposed by the prosecution, and as a result the case was referred to Iran's Revolutionary Court to settle the dispute, Iran's semi-official news agency ISNA reported.
The Revolutionary Court typically handles high-profile cases in Iran, including those involving espionage.
The university and supporters said this week that Adelkhah and another detained academic, Australian Kylie Moore-Gilbert, had started an indefinite hunger strike just before Christmas.
The French statement said the ministry had made clear to the ambassador "our grave concern over the situation of Mrs Fariba Adelkhah, who has stopped taking food".
"Creating hype cannot stop Iran's judiciary from handling the case, especially considering the security charges the two face," Mousavi said.
Mousavi had previously dismissed similar calls from France, saying it should remember that "Iran is sovereign and independent" and interference in its affairs is "unacceptable".
The latest tensions come after Xiyue Wang, an American scholar who had been serving 10 years on espionage charges, was released by Iran this month in exchange for Massoud Soleimani, an Iranian who had been held in the US for allegedly breaching sanctions.
Iran has said it is open to more such prisoner swaps with the United States.
Tehran is still holding several other foreign nationals in high profile cases, including British-Iranian mother Nazanin Zaghari-Ratcliffe and Iranian-American businessman Siamak Namazi and his father Mohammad Bagher Namazi.
US-Iran tensions have soared since Washington pulled out of a landmark nuclear agreement with Tehran last year and reimposed crippling sanctions.
Franco-Iranian academic Fariba Adelkhah, seen here in 2012, was arrested on charges of "espionage" that have been rubbished by her supporters