Greens senator Sarah Hanson-Young says she put up with sexist comments in parliament for years but the latest attacks from David Leyonhjelm have gone too far.
She wants to stamp out sexism in the workplace and has asked men around Australia to realise what women go through.
"If you see it happening to a woman, if you hear it happening to your sister, to your mother, to your work colleague, say something," an emotional Senator Hanson-Young told reporters on Tuesday.
"Stand up and say something because I can guarantee you she's not having fun.
"For years I have ignored it and pretended that they would just go away, that it wasn't impacting on me, that I couldn't hear the comments. I'm over it."
Senator Leyonhjelm told Senator Hanson-Young to "stop shagging men" during a debate in federal parliament last week.
He doubled down on those comments on Sunday before making "appalling" references to her private life in interviews on Sky News and Melbourne radio station 3AW.
Senator Hanson-Young has fired off legal letters to Senator Leyonhjelm, Sky News and 3AW but has not yet said if she will sue for defamation.
Prime Minister Malcolm Turnbull and Labor leader Bill Shorten called on Senator Leyonhjelm to apologise but he refused and lashed out instead.
"The prime minister should stop being such a pussy," Senator Leyonhjelm told SBS on Tuesday.
The NSW Liberal Democrat senator originally said he thought his Greens counterpart said "all men are rapists", but later admitted she said something like "men should stop raping women".
He argued it was not important that he got her word-for-word correct before verbally attacking her.
Senator Leyonhjelm has previously courted controversy with his views on the Port Arthur massacre, the Bourke St massacre and women's sport.