The Queensland Resources Council has doubled down on criticising the Greens after registering to run political ads at the election.
The QRC and the Greens have been feuding this week over negative state election ads singling out the minor party.
The controversial ads led to BHP and Origin suspending their memberships in the mining lobby.
QRC chief executive Ian Macfarlane redoubled his attacks on the Greens after applying to register his organisation as an official third party.
"The last thing Queensland needs is a one-issue party who have made it their priority to destroy the mining and gas sector," he said on Friday.
"The resources sector has been a life raft to Queensland during COVID-19 and it's completely irresponsible for any party to be publicly advocating for it to be shut down."
Greens MP Michael Berkman called for the electoral commission to prosecute the QRC for running election ads before it had officially registered.
"The QRC have effectively admitted they broke the law in running their dodgy campaign against the Greens," he said.
"The ECQ now must prosecute the QRC. The law is very clear, organisations must register and declare all electoral expenditure."
The anti-Green ads have spooked BHP, Origin, South32 and Anglo American, all of which say they would prefer the QRC to advocate policies rather than target a political party.
The Greens hold the seat of Maiwar in Queensland parliament and are pushing for two more - South Brisbane and McConnel - at the October 31 state election.
Their support could be vital for the next government and one of their signature policies is increasing mining royalties from $4.5 billion to $18 billion per year.
Mr Mcfarlane warned that even a preference vote for the Greens could damage the mining sector.
But Mr Berkman said hiking royalties had broad support and would fund more investment in health, education and renewables jobs.
"No amount of unlawful campaigning will stop us," he added.
Queenslanders go to the polls on October 31.