Germany has recorded more than 4000 coronavirus cases in 24-hours, a jump of more than 1200 on the previous day.
Numbers released on Thursday by the Robert Koch Institute showed 4058 new infections were reported, compared to 2828 on Wednesday.
The country has not recorded such a high number of daily cases since the height of the pandemic in late March and early April, when Germany was counting more than 6000 new cases per day before they fell off markedly.
Infections began trending upwards again in July.
On Wednesday, the majority of the nation's 16 federal states agreed to impose bans on people from a growing number of domestic hotspots from staying in hotels elsewhere in the country.
The discussion came as government spokesman Steffen Seibert said cases can no longer be assigned to a "single outbreak event" and warned health authorities trying to track infections could become overwhelmed.
The country has gradually lifted lockdown rules since May, as authorities opt for a contact-tracing strategy and localised restrictions in response to regional outbreaks.
Meanwhile, masks have become ubiquitous on public transport and in shops.
Germans are also being encouraged to keep windows open as summer draws to an end and socialising begins to move indoors.
Authorities in Berlin have also moved to clamp down on the German capital's famed nightlife after they came under fire from other states for their slow response to heading off the rise in cases.
At least 310,144 people in Germany have contracted the virus since the pandemic began and 9578 have died.