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Boris Johnson was booed as he walked into the Service of Thanksgiving for the Queen's Platinum Jubilee on Friday morning.
Crowds could be heard booing and whistling as the Prime Minister stepped out of his car with wife Carrie and walked up the steps to St Paul's Cathedral.
The Prime Minister appeared not to acknowledge the crowds, but the response to his arrival was audible on live footage covering the event with a sizeable proportion of the crowd jeering him.
The couple arrived at St Paul's Cathedral for the service shortly before members of the Royal Family.
The Duke and Duchess of Sussex arrived soon after and were loudly cheered by the crowd as they made their way into the building.
Neither the Queen or her son Prince Andrew were able to attend the event.
Buckingham Palace announced late on Thursday that the Queen would miss out due to experiencing "discomfort" at the Trooping the Colour parade on Thursday.
During the service, Johnson read a short exerpt from the Bible.
"Rejoice in the Lord always... Let your gentleness be evident to all...
"Do not be anxious about anything, but in every situation, by prayer and petition, with thanksgiving, present your requests to God."
Watch: Queen pulls out of thanksgiving service after experiencing "discomfort"