The pain of the Walsh Street killings is still raw in the minds of all Victorian police officers 25 years on, Chief Commissioner Ken Lay says.
Constable Steven Tynan, 22, and Constable Damian Eyre, 20, were gunned down on October 12, 1988, after being lured to an abandoned car in Walsh Street, South Yarra.
A memorial service to commemorate the 25th anniversary of the deaths was held at the nearby Prahran police station on Saturday.
Mr Lay said officers were still very conscious of the events of Walsh Street.
"The memories are still raw in the minds of all officers today," he said.
"Their deaths serve as a reminder of the dangers our officers face everyday, protecting and serving the Victorian community."
Mr Lay said the deaths sparked one of the longest investigations in the state's police history.
Victor Peirce, Peter McEvoy, Trevor Pettingill and Anthony Farrell were found not guilty of murder in 1991.A review of the case by the head of the homicide squad earlier this year found insufficient new evidence to warrant a retrial.